Zebrafish - Methods and Protocols

Thissecond edition details new emerging areas of zebrafish research focusing on geneticsand genomics, techniques for developing and analyzing zebrafish disease models,and methods for neuroscience. Zebrafish:Methods and Protocols, Second Edition guides readers through methods for mutagenesisand genome editing in zebrafish, applicationsof GFP-expressing transgenic fish, techniques for cancer models, imaging of infectionand host-pathogen interactions, metabolism and transport of lipids, and the structure and function of neural circuitsand their role in generating behavior. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.





Authoritative and practical Zebrafish: Methods and Protocols, Second Edition is a usefulcomple ment to the first book for new and experienced zebrafish researcheralike.

Inhaltsverzeichnis:

Chemical Screening in Zebrafish.- TALEN-MediatedMutagenesis and GenomeEditing.- HomologyIndependent Integration of Plasmid DNA into the Zebrafish Genome.- Detectionof Multiple Genome Modifications Induced by the CRISPR/Cas9 System.- Generation of Targeted Genomic Deletions Through CRISPR/Cas System in Zebrafish.- Testing of cis-Regulatory Elements by Targeted Transgene Integration inZebrafish using PhiC31 Integrase.- Fluorescence-Activated Cell Sorting and Gene Expression Profilingof GFP Positive Cells from Transgenic Zebrafish Lines.- Construction of the Inbred Strain.- Zebrafish as a Model for theStudy of Solid Malignancies.- Melanoma Regression and Recurrence in Zebrafish.- Imaging of Human Cancer Cell Proliferation, Invasion andMicrometastasis in a Zebrafish Xenogeneic Engraftment Model.- Modeling Leukemogenesis in the Zebrafish using Geneticand Xenograft Models.- EnumeratingHematopoietic Stem and Progenitor Cells in Zebrafish Embryos.- Live Imaging of Host-PathogenInterac tions in Zebrafish Larvae.- Methodologiesfor Inducing Cardiac Injury and Assaying Regeneration in Adult Zebrafish.- Studying Lipid Metabolism and Transport During ZebrafishDevelopment.- Targeted Electroporation in Embryonic, Larval and Adult Zebrafish.- Studying Axonal Regeneration by Laser Microsurgery and High-Resolution Videomicroscopy.- In Vivo Whole-Cell Patch-Clamp Recording in the Zebrafish Brain.- Quantifying Aggressive Behaviour in Zebrafish.- Correlating Whole Brain Neural Activity with Behavior in Head-Fixed Larval Zebrafish.- A Practical Guide to Light Sheet Microscopy.- Calcium Imaging of NeuronalActivity in Free-Swimming Larval Zebrafish.- Fiber Optic-Based Photostimulation of Larval Zebrafish.- Genetic Ablation, Sensitization and Isolation of Neurons Using Nitroreductaseand Tetrodotoxin-Insensitive Channels.