Enzyme Engineering - Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science

Inhaltsverzeichnis:

Introduction to Directed Evolution and Rational Design as Protein Engineering Techniques -Methods and Aims of Directed Enzyme Evolution -Short History of Directed Enzyme Evolution -Methods and Aims of Rational Design of EnzymesScreening and Selection Techniques Gene Mutagenesis MethodsGuidelines for Applying Gene Mutagenesis Methods in Organic Chemistry, Pharmaceutical Applications and BiotechnologyCase Studies of Protein Engineering of Activity and Selectivity -Epoxide Hydrolase -Transaminase as an Industrial Example with Pharmaceutical Application -Geranylgeranyl Diphosphate Synthase for Efficient Carotenoid Production -Cytochrome P450 Monooxygenases for Synthesis of Hydroxylation of Steroids Needed in the Preparation of Pharmaceuticals -Lipase for Stereocomplementary Production of Organic Compounds with Two Chirality Centers -Further Examples Using Other Enzyme TypesProtein Engineering of Enzyme Robustness -Examples of Relevance to Organic and Pharmaceutical Applications -Examp les of Relevance to BiotechnologyArtificial Metallo-Enzymes for Promiscuous Transformations Using Known Organic Reaction Types as a GuideLearning Lessons from Protein EngineeringPerspectives for Future Work -In Extending Applications in Organic and Pharmaceutical Chemistry -In Extending Biotechnological Contributions to Ecology

Klappentext:

Written by one of the pioneers, this book presents enzyme engineering and its applications at its best. Beside established topics like enzyme activity and selectivity, emerging fields like machine learning and AI are presented, too.

Inhaltsverzeichnis:

Introduction to Directed Evolution and Rational Design as Protein Engineering Techniques -Methods and Aims of Directed Enzyme Evolution -Short History of Directed Enzyme Evolution -Methods and Aims of Rational Design of EnzymesScreening and Selection Techniques Gene Mutagenesis MethodsGuidelines for Applying Gene Mutagenesis Methods in Organic Chemistry, Pharmaceutical Applications and BiotechnologyCase Studies of Protein Engineering of Activity and Selectivity -Epoxide Hydrolase -Transaminase as an Industrial Example with Pharmaceutical Application -Geranylgeranyl Diphosphate Synthase for Efficient Carotenoid Production -Cytochrome P450 Monooxygenases for Synthesis of Hydroxylation of Steroids Needed in the Preparation of Pharmaceuticals -Lipase for Stereocomplementary Production of Organic Compounds with Two Chirality Centers -Further Examples Using Other Enzyme TypesProtein Engineering of Enzyme Robustness -Examples of Relevance to Organic and Pharmaceutical Applications -Examp les of Relevance to BiotechnologyArtificial Metallo-Enzymes for Promiscuous Transformations Using Known Organic Reaction Types as a GuideLearning Lessons from Protein EngineeringPerspectives for Future Work -In Extending Applications in Organic and Pharmaceutical Chemistry -In Extending Biotechnological Contributions to Ecology